ExPASy Home page Site Map Search ExPASy Contact us PROSITE
Mirror sites:Australia Canada Taiwan

PROSITE: PDOC00787 (documentation)

View entry in NiceSite formatnew
{PDOC00787}
{PS01027; GLYCOSYL_HYDROL_F39}
{BEGIN}
******************************************************
* Glycosyl hydrolases family 39 putative active site *
******************************************************

It has  been  shown  [1,E1]  that  the  following  glycosyl  hydrolases can be
classified into a single family on the basis of sequence similarities:

 - Mammalian lysosomal alpha-L-iduronidase (EC 3.2.1.76).
 - Caldocellum  saccharolyticum  and  Thermoanaerobacter saccharolyticum beta-
   xylosidase (EC 3.2.1.37) (gene xynB).

The best  conserved  regions  in  these  enzymes is  located in the N-terminal
section. It   contains  a  glutamic  acid  residue  which,  on  the  basis  of
similarities with other  families of glycosyl hydrolases [2], probably acts as
the proton donor in the catalytic mechanism. We use this region as a signature
pattern.

-Consensus pattern: W-x-F-E-x-W-N-E-P-[DN]
                    [The second E is the putative active site residue]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in SWISS-PROT: NONE.

-Expert(s) to contact by email:
                   Henrissat B.; bernie@afmb.cnrs-mrs.fr

-Last update: November 1997 / Text revised.

[ 1] Henrissat B., Bairoch A.
     Biochem. J. 293:781-788(1993).
[ 2] Henrissat B., Callebaut I., Fabrega S., Lehn P., Mornon J.-P., Davies G.
     Proc. Natl. Acad. Sci. U.S.A. 92:7090-7094(1995).
[E1] http://www.expasy.ch/cgi-bin/lists?glycosid.txt

+----------------------------------------------------------------------------+
| This PROSITE entry  is copyright  by the Swiss Institute of Bioinformatics |
| (SIB).  There are no restrictions on its use by non-profit institutions as |
| long as its  content  is  in  no  way  modified  and this statement is not |
| removed. Usage by and for commercial entities requires a license agreement |
| (See http://www.isb-sib.ch/announce/ or email to license@isb-sib.ch).      |
+----------------------------------------------------------------------------+
{END}

ExPASy Home page Site Map Search ExPASy Contact us PROSITE
Mirror sites:Australia Canada Taiwan